Kisner, who boasts an impressive match-play record as a professional but had never been part of a Ryder Cup team, joins vice captains Webb Simpson and Brandt Snedeker on Bradley's staff.
"Kevin is a great addition to our leadership group and will be a trusted voice throughout the coming months and this year’s Ryder Cup," Bradley said in a news release.
"His extensive match-play success and strong relationships make him a perfect fit. I look forward to working with him closely as we get ready for Bethpage Black."
Kisner won the WGC-Dell Technologies Match Play in 2019 and finished runner-up in 2018 and 2022. Kisner also owns a 2-2-3 career record in three Presidents Cups.
Kisner served as a captain's assistant on the US team that secured a 10th consecutive Presidents Cup last September.
"I love match play and the Ryder Cup is one of the greatest events in sports," said Kisner.
"I cannot wait to be a part of it and look forward to doing everything I can to support Keegan, Webb, Brandt and our talented team in September."
Additional vice-captains will be announced later.
The Ryder Cup will be held from September 26th to 28th.