England play Norway in the quarter-finals in Miami on Saturday.
James has already missed three matches at the tournament with a hamstring injury suffered in England's 0-0 group-stage draw with Ghana on June 23.
Rice is managing a neural problem that troubled him towards the end of Arsenal's Premier League season and affects his hamstring and lower back. The midfielder was rested for England's 2-0 win over Panama in the group stage.
FIFA World Cup 2026
The 2026 World Cup took place from June 11th to July 19th in the United States, Canada and Mexico. The tournament featured 48 national teams and was won by Spain, who beat Argentina in the final.
Full schedule and results | Group standings and bracket | Past winners of the tournament | Our team of the tournament | About the 2030 World Cup
England defender Jarell Quansah is already set to miss Saturday's clash with Norway after his sending-off during England's dramatic 3-2 victory over Mexico in the last 16 at the Azteca Stadium on Sunday.
Rice, Guehi, Harry Kane, Jude Bellingham and Nico O'Reilly are also all walking a tightrope on yellow cards, with another booking automatically ruling them out of a potential semi-final against Argentina or Switzerland.