The squad, released by the Spanish soccer federation (RFEF) on Wednesday, includes 34-year-old all-time top scorer Jennifer Hermoso and Ballon d'Or winners Alexia Putellas and Aitana Bonmati.
Hermoso, Bonmati and Ona Batlle scored in Spain's 3-0 home win over the Netherlands in the Nations League semi-finals in February that secured Olympic qualification for the first time.
Spain are drawn in Group C at Paris along with Japan, Nigeria and Brazil. The women's football tournament will be held from July 25 to Aug. 10.